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Inhibition of glutathione metabolism by BSO decreased the ESCC tumor burden To investigate whether glutathione metabolism contributed to the progression of ESCC development, we treated mice bearing ESCC 20 weeks after initial carcinogen treatment with buthionine sulfoximine (BSO), a commonly used chemical inhibitor of -glutamylcysteine synthetase (-GCS) that decreases tissue glutathione concentrations (Figure 3a)

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